A54 may refer to:
- A54 road (England), a road connecting Chester and Buxton
 - A54 motorway (France), a road connecting Salon-de-Provence and Nîmes
 - A54 motorway (Italy), a ring road around Pavia
 - A-54 freeway (Spain), a road connecting Santiago de Compostela and the N-550, N-634 and N-547
 - A54 motorway (Belgium), a road connecting Charleroi and E19
 - Agent A-54, a German double agent during World War II
 - Old Indian Defense, Encyclopaedia of Chess Openings code
 - Fiat A.54, a seven-cylinder, air-cooled radial engine developed in Italy in the 1930s as a powerplant for aircraft
 - INS Amba (A54), a 1968 Indian Navy submarine tender ship
 - Kanehana Station in Hokkaidō, Japan
 - Samsung Galaxy A54 5G, an Android smartphone by Samsung Electronics
 
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