The following highways are numbered 129:

Canada

• Ontario Highway 129
• Prince Edward Island Route 129

Costa Rica

• National Route 129

India

• National Highway 129 (India)

Japan

• Japan National Route 129

United States

• Interstate 129
• U.S. Route 129
• Alabama State Route 129
• Arkansas Highway 129
• California State Route 129
• Florida State Road 129
  • County Road 129 (Columbia County, Florida)
• Georgia State Route 129
• Illinois Route 129
• Indiana State Road 129
• K-129 (disambiguation) (former), two former highways
• Kentucky Route 129
• Louisiana Highway 129
• Maine State Route 129
• Maryland Route 129
• Massachusetts Route 129
  • Massachusetts Route 129A
• M-129 (Michigan highway)
• Missouri Route 129
• New Hampshire Route 129
• New Jersey Route 129
• New Mexico State Road 129
• New York State Route 129
  • County Route 129 (Jefferson County, New York)
  • County Route 129 (Monroe County, New York)
  • County Route 129 (Niagara County, New York)
  • County Route 129 (Seneca County, New York)
  • County Route 129 (Westchester County, New York)
• Ohio State Route 129
• Pennsylvania Route 129 (former)
• South Carolina Highway 129
• Tennessee State Route 129
• Texas State Highway 129 (former)
  • Texas State Highway Spur 129
  • Farm to Market Road 129
• Utah State Route 129 (disambiguation), several former highways and a current one
• Vermont Route 129
• Virginia State Route 129
  • Virginia State Route 129 (1928-1933) (former)
• Washington State Route 129
• West Virginia Route 129
• Wisconsin Highway 129

Territories

• Puerto Rico Highway 129