Route 240 or Highway 240 may refer to:

Australia

• Wimmera Highway

Canada

• Manitoba Provincial Road 240
• Prince Edward Island Route 240
• Saskatchewan Highway 240

Costa Rica

• National Route 240

Japan

• Japan National Route 240

United States

• Interstate 240
• U.S. Route 240 (former)
• California State Route 240 (former)
• Florida State Road 240 (former)
• Georgia State Route 240
• Hawaii Route 240
• Indiana State Road 240
• Kentucky Route 240
• Massachusetts Route 240
• Minnesota State Highway 240 (former)
• Missouri Route 240
• Montana Secondary Highway 240
• New Mexico State Road 240
• New York State Route 240
• Oregon Route 240
• Pennsylvania Route 240
• South Dakota Highway 240
• Tennessee State Route 240
• Texas State Highway 240
  • Texas State Highway Loop 240
  • Texas State Highway Spur 240
• Utah State Route 240
• Virginia State Route 240
• Washington State Route 240
• Wyoming Highway 240