Margulies (occasionally Margolies or Margolyes) is a surname that, like its variants shown below, is derived from the Ashkenazi Hebrew pronunciation of the Hebrew word מרגליות (Israeli Hebrew [maʁɡaliˈjot]), meaning 'pearls'. Notable people with the surname include:
- Ben Margulies, American songwriter and record producer
 - David Margulies (1937–2016), American actor
 - Donald Margulies (born 1954), American playwright
 - Jimmy Margulies (born 1951), American award-winning editorial cartoonist
 - Joseph Margulies (artist) (1896–1984), Vienna-born American painter and printmaker
 - Joseph Margulies (lawyer), American attorney and law professor
 - Julianna Margulies (born 1966), American actress
 - Lazar C. Margulies (1895–1982), physician and inventor of a type of Intrauterine device
 - Leo Margulies (1900–1975), American editor and publisher
 - Martin Margulies (born 1948), American musician/film producer better known as Johnny Legend
 - Martin Margulies , real-estate developer and collector of contemporary art and photography
 - Roni Margulies (1955–2023), Turkish poet and activist
 - Samuel Hirsch Margulies (1858–1922), rabbi and Jewish scholar
 - Susan Margulies, American engineer and professor
 - Miriam Margolyes (born 1941), British-Australian actress
 
See also
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