Spen may refer to:
- SPEN, human gene
 - S.P.E.N., Secret sof
 - Spen, West Yorkshire, a location in England
 - River Spen, river in West Yorkshire, England, the United Kingdom
 - SS Spen, British steamship built in 1908
 - Spen Whittaker (1871–1910), English football manager
 - SP Energy Networks, the energy networks arm of Scottish Power
 
See also
- S Pen, stylus pen for Samsung smartphones
 
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